Analysis of Microarray Data using ASReml

Hi ASReml Users,

I am working on a gene expression (microarray) data set of 398000
observations. I fitted the following mixed model to normalize data.
log2i ~ mu dye !r slide slide*dye
The model it self is not complicated, but ASReml failed to finish the
analysis. I increased the memory allocation 3 fold (-S3). That did not
help either. I received the following error message: "Severe <157>,
Program Exception-access violation". Is there a way to overcome this
problem? Can ASReml handle this kind of large data sets?'.

My second questions is about fitting a gene-by-gene model using the
normalized data. In SAS this can be done using the 'BY' statement before
the model statements as follows.

proc mixed data=&ds cl lognote;
 BY geneid ;
     class dye slide sample;
     model log2in=dye sample/outp=array.gene_res ;
     random slide;
run;

SAS runs the model for each gene separately. However, in general, if the
data are large, the log message window overflows and it takes quite a
long time to finish the job. Is there a way to fit the same model in
ASReml for multiple genes each time for a different gene and to automate
the job? Any comments will be appreciated.
Thanks,

Fikret Isik

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Fikret Isik, Research Assistant Professor
North Carolina State University
Department of Forestry,
Campus Box 8002, Raleigh, NC 27695, USA
Phone 919-515-5029, Fax 919-515-3169
http://www4.ncsu.edu/~fisik
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